To observe the effects of Haoqin-Huaban on the relative adhesion factors and the PI3K/AKT/GSK-3β pathway of an acute UVB light HaCat-cell injury model in order to further explore the mechanism of the treatment for photosensitive skin disorders.Methods:UVB light(300 mJ/cm2) was used to establish an acute light HaCat-cell injury model.The intervention was performed by using Haoqin-Huaban in various concentrations.The groups were divided into：a blank control group(C); an UVB model group(M); a high Haoqin-Huaban dosage group(G); medium Haoqin-Huaban dosage group(Z); low Haoqin-Huaban dosage group(D).Real-time PCR was conducted to detect the relative expressions of 3 cell adhesion molecules(CAMs)，i.e.，ICAM-1，ELAM-1 and VCAM-1.Western Blotting was implemented to analyze the relative expressions of p-AKT(Ser-473)，p-AKT(Thr-308)，GSK-3β，and PI3K，which were all related nodes of the PI3K/AKT/GSK-3β pathway.Western Blot method was used to analyze the relative expression of p-AKT(Ser-473)，p-AKT(Thr-308)，GSK-3β and PI3K related nodes in the PI3K/AKT/GSK-3β pathway.Results:UVB acute light injury reduced ICAM-1 expression in the HaCat cell model and had no significant effect on the expressions of VCAM-1 and ELAM-1(P<0.01).The expressions of the three CAMs in the HaCat cell model were effectively inhibited in the G group(P<0.05)，while those of ELAM-1 and VCAM-1 were considerably impeded in the Z group(P<0.01).It was also found that acute UVB light injury had no significant effect on AKT phosphorylation and PI3K expression in the HaCat cell model.The low-concentration treatment of Haoqin Huaban Forumla can promote the phosphorylation of AKT in HaCat cells(P<0.05).Acute UVB light damage increased the GSK-3β expression level of HaCat cells(P<0.01).The high，medium and low concentration treatments of Haoqin Huaban Formula inhibited the GSK-3β expression level of HaCat cells(P<0.01).Conclusion:Haoqin-Huaban Formula can reduce the expression of adhesion molecules such as ICAM-1，ELAM-1 and VCAM-1 in HaCat cells caused by acute UVB damage，thereby alleviating the occurrence of local inflammation and delayed-type hypersensitivity，and can activate HaCat cells PI3K/AKT/GSK-3β pathway and plays a role in anti-oxidation，anti-apoptosis and reducing inflammation in the acute light damage caused by UVB.