To investigate the effects of total glucosides of paeony(TGP) on the proliferation，apoptosis and inflammatory response of fibroblast synovial cells in rheumatoid arthritis and its mechanism.Methods:Human fibroblast-like synoviocytes(HFLS-RA) were divided into a control group，a model group and an intervention group randomly，and the cells of the control group were cultured routinely.Cells in the model group and intervention group were cultured with tumor necrosis factor(TNF)-α 10 ng/mL to establish RA cell models in vitro.Subsequently，cells in the intervention group were cultured with TGP 62.5 μg/mL，and cells in the control group and model group were cultured with the same amount of normal saline.CCK-8 assay method was used to detect cell proliferation.Cell apoptosis rate was detected by in situ end transferase labeling(TUNEL).The levels of interleukin(IL)-6 and vascular endothelial growth factor(VEGF) were determined by enzyme-linked immunosorbent assay(ELISA).The expression levels of phosphorylated JAK2(p-JAK2)，phosphorylated STAT1(p-STAT1) and phosphorylated STAT3(p-STAT3) were detected by Western blotting.Results:CCK-8 and TUNEL assay showed that TGP could inhibit the proliferation of HFLS-RA cells and promote cell apoptosis.ELISA assay showed that TGP can reduce the levels of IL-6 and VEGF in cells.Western blotting assay showed that TGP can reduce the expression levels of p-JAK2，p-STAT1 and p-STAT3(P<0.05).Conclusion:TGP can inhibit HFLS-RA cell proliferation，promote cell apoptosis，inhibit synovial cell inflammation and angiogenesis by regulating JAK/STAT signaling pathway.