To investigate the effect of cannabinoid 1 receptor(CB1R) overexpression on cerebral nerve stem cells in mice with experimental autoimmune encephalomyelitis.Methods:EAE models(50 mice) were established,as well as an adjuvant group of 10 and a control group of 10 mice.In the models built successfully,EAE mice were injected with CB1R overexpression lentivirus and recorded as CBlR overexpression group(20 mice).Null vector group(10 mice) and the EAE model group(20 mice) were also set.Neurological functions of mice in each group were evaluated,pathological changes of brain tissue were observed,and levels of CB1R,Brdu and Brdu+/Nestin+ in subependymal area and SOX-2 mRNA of brain tissue were detected.Results:In the EAE model group,neurological function score increased and CBlR protein decreased(P<0.05),while neurological function score decreased and CBlR protein increased in the CBlR overexpression group(P<0.05).Typical pathological changes,such as inflammatory infiltration and myelin loss,occurred in the EAE model group.Pathological damage was significantly improved in the CBlR overexpression group.The levels of Brdu,Brdu+/Nestin+ positive cells and SOX-2 mRNA in the EAE model group were significantly increased(P<0.05),and so were the other two groups(P<0.05).Conclusion:Overexpression of CBlR may promote the repair of brain tissue damage by accelerating the proliferation of brain neural stem cells in mice,and effectively alleviate the symptoms of autoimmune encephalomyelitis in the end.