To analyze metabolites related to the progression of diabetic nephropathy and their molecular targets and mechanisms of action based on network pharmacology technology.Methods:Metabolites that were highly correlated with the progression of diabetic nephropathy were screened out based on early metabolomics.KEGG and PubChem databases were used to retrieve and extract the effective target genes of metabolization and conduct pathway analysis; Cytoscape 3.2.1 software ClueGO were used to conduct enrichment analysis of pathways,biological processes,cell components,and molecular functions of metabolite gene targets,and we drew network diagrams.Results:A total of 31 metabolites related to the progression of diabetic nephropathy were analyzed in the study.By using the KEGG and PubChem databases,a total of 31 metabolites were extracted,involving 82 metabolic pathways and 252 gene targets; the gene targets of metabolites involved a total of 157 pathways.Metabolite gene targets were mainly concentrated in metabolites such as glutamate,tryptophan,and phenylalanine.Analysis based on the core nodes of the metabolite-gene target network diagram,suggests that glutamate was the most connected node,followed by tyrosine,glutamine,tryptophan,etc.; the most connected pathways included glycolysis/sugar Xenobiotics,fatty acid degradation,amino acid synthesis,etc.; the most concentrated gene targets were NOS2,NOS3,ALDH7A1,etc.The enrichment analysis results of gene targets showed that metabolite gene targets were mainly concentrated in amino acid metabolism,fatty acid,nitrogen metabolism etc.Analysis of the cellular components involved in gene targets suggestd that the progression of diabetic nephropathy was mainly related to the abnormalities of mitochondria,synaptic membranes,presynaptic membranes and other cellular parts.The molecular functions involved in disease progression included amino acids,carboxylic acids,and vitamin splicing.Conclusion:Through in-depth network analysis of metabolite gene targets,it is suggested that abnormal amino acid metabolism is very important in the progression of diabetic nephropathy and is a key node involved in the regulation of immune metabolism of the body.