To explore the molecular mechanism of Guizhi Fuling Pills in the treatment of uterine fibroids by using bioinformatics technology.Methods:The uterine fibroids related chips were searched through the NCBI GEO database,and differentially expressed genes were obtained by R language analysis.Search for known target genes of uterine fibroids with the aid of the disease database.We used the TCM System Pharmacology Database and Analysis Platform (TCMSP) to screen the main active ingredients and target genes of Guizhi Fuling Pills.The 3 sets of target genes mapped out the key targets,and gene function annotation database (DAVID) was used to gene the key target genes,Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis.Results:A total of 11 active ingredients of Guizhi Fuling Pills were screened,corresponding to 121 targets; 187 differentially expressed genes were obtained from the GEO database,and 271 targets related to uterine fibroids were retrieved; the 3 sets of targets finally obtained 20 mapped target genes,key targets 9 genes.Guizhi Fuling Pills mainly regulated biological processes such as steroid binding and zinc ion binding through core target genes such as ESR1,EGFR,and SRC,as well as multiple signaling pathways such as estrogen pathway,prolactin pathway,and GnRH pathway to intervene in the occurrence and development of uterine fibroids.Conclusion:The application of bioinformatics has a high degree of confidence and reference value,and provides new directions and new ideas for Guizhi Fuling Pills in the treatment of uterine fibroids.