To observe the effects of Tongfengning on uric acid induced THP-1 inflammation to reveal the optimal time of intervention.Methods:THP-1 was randomly divided into an MSU model establish group and a blank control group.The former received MSU suspension to establish the cell model of inflammation，and the latter received no intervention.After the successful identification of the model，THP-1 in the MSU model establish group was randomly divided into a model group，a Tongfengning group and a positive control group and received 10% blank serum，10% serum containing Tongfengning，10% blank serum+Caspase-1 inhibitor for intervention,respectively.The blank control group received 10% blank serum intervention.At 6，12，24，48 h after the intervention，the expression of NALP3，Caspase-1，ASC protein，mRNA and the content of IL-1β and PGE2 in THP-1 in each group were detected.Results:At all point-in-time，compared with the blank control group，the protein and mRNA expression of NALP3，Caspase-1，ASC and the levels of IL-1β，PGE2 in the model group were higher(P<0.01 or P<0.05).At all point-in-time，the levels of all indexes in Tongfengning group were lower than the model group(P<0.01 or P<0.05).The levels of IL-1β，PGE2 and the expression of Caspase-1 protein in the positive control group at each point-in-time were significantly lower than those in the model group(P<0.01 or P<0.05)，and there was no difference in the expression of NALP3，ASC protein and mRNA.Compared with the positive control group，the indexes of Tongfengning group showed significant differences(Ps<0.05)at 12，24，48 hours after intervention.There were differences between the expressions of indexes in Tongfengning group at different point-in-time(P<0.01 or P<0.05).When Tongfengning intervened for 24 hours for the first time，the expression levels of inflammasome-related proteins，mRNA and inflammatory factors reached the lowest.Conclusion:Tongfengning intervenes THP-1 stimulated by MSU for 6，12，24 and 48 hours showed significant effect of inhibiting inflammatory response.Especially the anti-inflammatory effect reaches a peak at 24 hours after the first intervention.