Based on liver X receptors/nuclear factor-kappa B signaling pathway，the effects of Zhuanggu Jianxi Formula on the synovitis model of rabbit knee osteoarthritis were observed，and the effects and mechanism of Zhuanggu Jianxi Formula on synovitis were discussed.Methods:A total of 24 New Zealand white rabbits were randomly divided into a normal group(n=6)and a model group(n=18).The synovitis model of knee osteoarthritis in rabbits was established by intraarticular injection of papain.After successful modeling，the model rabbits were randomly divided into a model group，a voltaren group and a Zhuanggu Jianxi Formula group，with 6 rabbits in each group.Voltaren group and Zhuanggu Jianxi Formula group were given voltaren suspension and Zhuanggu Jianxi Formula by gavage，and the model group and normal group were given the same amount of normal saline by gavage.The skin temperature，circumference and Lequesne MG scores of the knee joint and the contents of IL-1β，TNF-α，MMP-3，MMP-13 in knee fluid and the synovial tissue score，the expressions of LXRα，N-COR，P50，p65 mRNA in synovial tissue were observed before and after intervention.Results:Compared with the normal group，the rabbits in the model group had a worse general condition，the skin temperature，circumference，Lequesne MG scores of the knee joint，the contents of IL-1β，TNF-α，MMP-3，MMP-13 in knee fluid，synovial tissue score，expressions of P50 and P65 mRNA were significantly increased in model group(Ps＜0.05)，while the expressions of LXRα and N-CoR mRNA in synovial tissue were decreased(Ps＜0.05).Compared with the model group，the knee joint skin temperature，circumference，Lequesne MG score，the content of IL-1β，TNF-α，MMP-3，MMP-13 in the knee joint fluid，synovial tissue in the Zhuanggu Jianxi Formula group and the voltaren group score，P50 and P65 mRNA expression were all low(Ps＜0.05).LXRα and N-CoR mRNA expression in Zhuanggu Jianxi Formula group were increased(Ps＜0.05)，and there was no statistical difference in LXRα and N-CoR mRNA expression in voltaren group(Ps＞0.05).Compared with the voltarin group，the Zhuanggu Jianxi Formula group increased the expression of LXRα and N-CoR mRNA(Ps＜0.05)，and the joint circumference was smaller(P<0.05).There was no statistically significant difference in other indicators(Ps＞0.05).Conclusion:Zhuanggu Jianxi Formula may inhibit the synovitis response of knee osteoarthritis by regulating the transmission of liver X receptors/nuclear factor-kappa B pathway and reducing the secretion of downstream inflammatory cytokines.