The unique small size effect and surface effect of nanoparticles make silver nanoparticles have broad-spectrum antibacterial activity.However,too high concentration of silver nanoparticles may cause toxicity or damage to other cells or organs.Therefore,in order to better exert antibacterial activity,it is extremely necessary to analyze silver nanoparticles quickly and accurately.Methods:By optimizing and screening the nano-silver particle adsorption method and paper-based chip,the laser-induced breakdown spectra of nano-silver particles were measured.The qualitative analysis of nano-silver particles was carried out by comparing the characteristic peak position with the standard spectral library,and the quantitative model of nano-silver particle analysis was established by the linear regression relationship between the characteristic spectral intensity and the concentration of nano-silver.Results:By repeating the “dipping-drying” process three times,the nano-silver particles adsorbed on the surface of the quantitative filter paper showed characteristic peaks of silver at 328 nm,338 nm,520 nm,546 nm and 827 nm.In the concentration range of 312.5～5 000 μg/mL,the characteristic peak intensity of nano-silver at the wavelength of 520 nm had a good linear relationship with the concentration (r=0.998 8).Conclusion:The LIBS analysis technology can be used for qualitative and quantitative analysis of nano-silver.The analysis method is accurate and sensitive,and the detection process is convenient and convenient,which can be easily used in practice.