To explore the main active components and potential mechanism of Buzhong Yiqi Pills in anti-fatigue.Methods:The potential active components in Buzhong Yiqi Pills were screened according to TCMSP database.Swiss target Prediction,drugbank,Cytoscape 3.7.1,R language and other databases and softwares were used to predict the target of active ingredients and fatigue related targets,to construct the interaction network of disease-drug-active-ingredients target genes and PPI network of protein interaction,and to carry out enrichment analysis of GO and KEGG of key target genes.Finally,animal experiments were designed to explore the anti-fatigue effect of Buzhong Yiqi Pills.Results:The results showed that 163 potential active components related to Buzhong Yiqi Pills could act on 108 targets and 160 pathways related to fatigue.Further analysis of the network characteristics showed that the main active components were flavonoids and sterols,which regulated MAPK,PI3K AKT,TNF and other signaling pathways through participating in lipopolysaccharide response,the reaction of source bacterial molecules and other biological processes,thus playing an anti-fatigue role.The results of pharmacodynamics test showed that Buzhong Yiqi Pills could significantly enhance the exercise endurance of mice,but had no significant effect on relieving physical fatigue.Conclusion:Buzhong Yiqi Pills gets the anti-fatigue characteristics of multi-component,multi-target and multi-channel.Its active components can regulate multiple biological processes and signal pathways by interacting with multiple target proteins,which can improve the body's oxidative stress response and regulate cell function.However,it has no obvious enrichment effect and pharmacodynamic results in regulating the body's energy metabolism.This study provides theoretical guidance for the further development of anti-fatigue functional products and drugs and clinical application,and provides new ideas for exploring the anti-fatigue mechanism of Buzhong Yiqi Pills.