To determine the content of Polysaccharide in Lycium ruthenicum Murr.using 1H nuclear magnetic resonance spectroscopy quantitively.Methods:MRI spectrometry and Bruker Advanced Ⅲ.600 MRI spectrometer were used，with deuterium oxide used as solvent.With relaxation time(D1)as 1 s，pulse width(P1)as 14.90 s，number of scans(NS)as 32，the nuclear magnetic resonance hydrogen spectrum was collected.With the hydrogen proton of shunbutene acid with a chemical shift(δ)of 6.40 as the internal standard peak，and the twin peaks at glucose chemical shift(δ)at 5.22~5.23 and 4.62~4.64 as quantitative peaks，glucose content was calculated.Results:The content of polysaccharide in Lycium ruthenicum Murr.was accurate and reliable by this method.Conclusion:The analysis shows that this method can be used for quantitative analysis of polysaccharie in Lycium ruthenicum Murr.，which has the advantages of rapidness，accuracy and convenience.