To compare the protective effects of wine-processed and raw Siegesbeckiae Herba on H9c2 myocardial cells induced by hypoxia and explore the underlying mechanism to provide the experimental basis for clinical drug selection.Methods:Hypoxia-induced H9c2 cells were treated by wine-processed and raw Siegesbeckiae Herba of different concentrations.Cell viability was detected by Cell Counting Kit-8(CCK-8) and the lactic dehydrogenase(LDH) activity in cell culture supernatant by colorimetry.Superoxide dismutase(SOD) activity was also detected.The fluorescence microplate reader was used to detect the content of intracellular reactive oxygen species(ROS).Western blot was used to detect the protein expression of B-cell lymphoma 2(Bcl-2) and Bcl-2-associated X protein(Bax).Results:The hypoxia model was properly induced in H9c2 myocardial cells after 16 hours of hypoxia.The screening results revealed that the optimal concentration of wine-processed and raw Siegesbeckiae Herba was 300 μg/mL.Compared with the conditions in the model group,wine-processed and raw Siegesbeckiae Herba could improve cell viability(P<0.05),improve damaged cell morphology,reduce LDH leakage(P<0.05),decrease ROS content(P<0.05),potentiate SOD activity(P<0.05),and up-regulate the expression of Bcl-2(P<0.05) and Bcl-2/Bax.In the context of administration concentration of 300 μg/mL,raw Siegesbeckiae Herba was superior to wine-processed Siegesbeckiae Herba in potentiating SOD activity and increasing cell viability(P<0.05),while wine-processed Siegesbeckiae Herba was superior to raw Siegesbeckiae Herba in inhibiting LDH leakage.No statistically significant difference in ROS content and protein expression of Bcl and Bax was observed between wine-processed and raw Siegesbeckiae Herba.Conclusion:Wine-processed and raw Siegesbeckiae Herba possessed protective effects on H9c2 myocardial cells induced by hypoxia,and the mechanism might be related to the resistance to oxidative stress and the inhibition of myocardial apoptosis.