To construct Carthamus tinctorius medicinal material composition-target-pathway network and to explore the mechanism of Carthamus tinctorius in the treatment of myocardial ischemia.Methods:The compounds in Carthamus tinctorius which met OB≥30%,DL≥0.18 were taken as the research objects.The components and targets which met the requirements were searched by TCMSP Database，and the gene names of the targets were searched in UniProt Database.The myocardial ischemia targets were obtained by searching for myocardial ischemia in GeneCards Database.The intersection of myocardial ischemia target and Carthamus tinctorius target was obtained.The intersection targets were analyzed by PPI and GO and KEGG pathways.Cytoscape 3.8.0 software was used to draw the network diagram of medicinal drug-compound-target-pathway.Results:There were 16 active compounds and 192 targets in Carthamus tinctorius，and 335 targets of myocardial ischemia were obtained by searching for myocardial ischemia in GeneCards.Taking Carthamus tinctorius target and myocardial ischemia target as intersection，58 intersection targets were obtained.PPI analysis was performed on the intersection targets，and the top 3 targets with PPI score were:IL6，TNF，and VEGFA.The enrichment analysis of GO and KEGG pathways selected the first 5 entries to draw bubble charts.There were 89 nodes and 338 edges in the medicinal material-compound-target-pathway network.The top 3 compounds at the node were:quercetin，kaempferol and luteolin.Conclusion:Carthamus tinctorius may play a role in the treatment of myocardial ischemia through various chemical components，multiple targets and multiple ways，which provides theoretical basis for its clinical use.