To screen key genes of Helicobacter pylori(Hp)-related chronic atrophic gastritis based on biomedical big data and explore its pathogenesis and the influence of Anwei Decoction.Methods:The chip data of Hp-related chronic atrophic gastritis were downloaded from Gene Expression Omnibus(GEO)，and the differential genes of HP-related chronic atrophic gastritis were screened out by GEO2R.Bioinformatics analysis was conducted，and the core proteins were determined according to the number of protein-participating pathways.The Hp(+) chronic atrophic gastritis model was induced in rats.PCR and Western blot were used to detect the effect of Anwei Decoction on the expression.Results:After retrieval and analysis of GSE13873 and GSE27411 series chip data，20 common differential genes of the two chips were taken for bioinformatics analysis，and the results showed that apolipoprotein A1(APOA1)，CD36，and cystic fibrosis transmembrane conductance regulator(CFTR) might be the core proteins of the signaling pathway.Western blot and PCR results showed that the expression of CD36 protein was down-regulated(P<0.05)，and the expression of APOA1 and CFTR was up-regulated(P<0.05) in the gastric tissues of rats with HP-related chronic atrophic gastritis.Compared with the model group，the high-，medium-and low-dose Anwei Decoction groups showed up-regulated expression of CD36 and down-regulated expression of APOA1 and CFTR(P<0.05)，which was closely related to the incidence of HP-related chronic atrophic gastritis.Conclusion:As revealed by the bioinformatics analysis of HP-related chronic atrophic gastritis in GEO microarray and verification by Western blot and PCR，the experimental results of APOA1，CD36，and CFTR as the core proteins of the signaling pathway were consistent with the results of bioinformatics analysis.