To analyze the acupoint selection regularity in modern research on acupuncture in the treatment of premature ovarian insufficiency(POI) based on data mining.Methods:The research articles related to acupuncture treatment of POI published from database inception to March 31，2021，were retrieved from CNKI，CSPD，CCD，PubMed，Cochrane Library，and EMbase.The acupoint information in prescriptions was collected，and data mining was performed by the Traditional Chinese Medicine Inheritance Support System(TCMISS，V2.5).Results:Seventy-eight research articles were included，involving 64 acupoints with a cumulative frequency of 478 times acting on bladder meridian，conception vessel，and spleen meridian.The most commonly used acupoints in acupuncture treatment of POI included Guanyuan(CV 4)，Sanyinjiao(SP 6)，Shenshu(BL 23)，uterus(EX-CA 1)，Zusanli(ST 36)，Taichong(LR 3)，Zhongji(CV 3)，Xuehai(SP 10)，Pishu(BL 20)，and Ciliao(BL 32).The application of specific acupoints，such as crossing points and alarm points，was highlighted.The commonly used combination of acupoints is Guanyuan(CV 4)-Sanyinjiao(SP 6)-Shenshu(BL 23).Based on the entropy clustering algorithm，nine candidate new acupoint combinations and two new acupoint prescriptions were obtained.Conclusion:The meridians in acupoint selection of acupuncture treatment of POI are mainly bladder meridian，conception vessel，and spleen meridian，and the core acupoint combination is Guanyuan(CV 4)-Sanyinjiao(SP 6)-Shenshu(BL 23).The acupuncture treatment of POI should follow the principles of regulating thoroughfare and conception vessels，tonifying the liver and the kidney，regulating qi and blood，and tranquilizing the mind.