To observe the effects of acupuncture on the hind paw mechanical pain threshold and chemokine receptor 2(CCL2) and chemokine(C-X-C motif) ligand 1(CXCL1) in the spinal trigeminal nucleus caudalis(Sp5C) in rats with chronic migraine(CM)，so as to provide a reasonable basis for clinical application of acupuncture in the treatment of migraine.Methods:Twenty-eight rats were randomly divided into a control group，a model group，an acupuncture group，and a non-meridian and non-acupoint group，with 7 rats in each group.The rat model of CM was established by repeated subcutaneous injection of nitroglycerin(NTG) at the neck and back every other day.The acupuncture group and the non-meridian and non-acupoint group were treated with acupuncture every day for 20 min.The the hind paw mechanical pain threshold of rats was measured by Von Frey instrument.The content of CCL2 and CXCL1 in the Sp5C was determined by enzyme-linked immunosorbent assay(ELISA).Results:As compared with the control group，the hind paw mechanical pain threshold of rats in the model group was significantly decreased(P<0.01).As compared with the model group，the hind paw mechanical pain threshold of rats in the acupuncture group was significantly increased(P<0.01).As compared with the control group，the content of CCL2 and CXCL1 in the Sp5C increased in the model group(P<0.01).The content of CCL2 and CXCL1 in the acupuncture group was lower than that in the model group(P<0.01).Conclusion:Acupuncture can reduce the pain degree of migraine by down-regulating the expression of chemokines CCL2 and CXCL1，thereby playing a therapeutic role in migraine.