To predict the anti-fibrosis mechanism of absorbed components of Panax notoginseng saponins(PNS),and to explore its effective substances and action pathways by network pharmacology and molecular docking.Methods:Absorbed components of PNS were detected and determined as potential active components,and their potential action targets were screened by PharmMapper combined with CooLGeN and GeneCards.Gene Ontology(GO) function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were carried out through DAVID.Protein-protein interaction(PPI) network and absorbed component-target-pathway-disease network were plotted using STRING and Cytoscape.Molecular docking between absorbed components and potential key targets were conducted by Autodock Vina.Results:Twenty absorbed components of PNS were determined,and 31 potential targets for the treatment of renal fibrosis were obtained,involving biological processes such as intracellular signal transduction,apoptosis,proliferation,matrix decomposition and hypoxia response,as well as eight significantly related signal pathways such as phosphoinositide-3-kinase/protein kinase B(PI3K/Akt),Ras-associated protein 1(Rap1),hypoxia-inducible factor-1(HIF-1) and forkhead box O(FOXO) signal pathways.Network analysis showed that AKT1,SRC,CASP3,NOS2 and EGFR were the key targets of anti-renal fibrosis.Molecular docking indicated that ginsenoside Rh2,malonyl ginsenoside Rb1,ginsenoside Rb1,ginsenoside Rg2,ginsenoside Rb3,ginsenoside Rb2 and ginsenoside Rg3 had strong binding activity to AKT1,SRC,CASP3 and NOS2.Conclusion:This study preliminarily revealed the multi-component,multi-target and multi-channel mechanism of PNS in the treatment of renal fibrosis,which provided new ideas and scientific basis for subsequent in-depth pharmacological research and clinical development.