To predict the targets and signaling pathways of Bushen Formula so as to explore the possible mechanism of the formula in the treatment of Alzheimer's disease(AD).Methods:The active components and targets of Bushen Formula were searched against the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and Bioinformatics Analysis Tool for Molecular mechANism of TCM(BATMAN-TCM),and the genes corresponding to the targets were collected from Uniprot.The targets of AD were obtained from GeneCards.Venny 2.1 was used to identify the common targets shared by Bushen Formula and AD.A protein-protein interaction(PPI) network was established via String.Metascape was used to perform gene ontology(GO) annotation and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment.Cytoscape 3.7.1 was used to perform visual analysis.Results:Bushen Formula contained four herbs,which corresponded to 45 active components and 397 targets.Bushen Formula and AD shared 48 common targets,for which 1 077 GO terms(P<0.01) and 128 KEGG signaling pathways(P<0.01) were enriched.Conclusion:The key targets of Bushen Formula for treating AD include AKT serine/threonine kinase 1(AKT1),interleukin-6(IL-6),caspase 3(CASP3),tumor necrosis factor(TNF),and tumor protein p53(TP53).These targets participate cooperatively in multiple signaling pathways,such as IL-17 and hypoxia-inducible factor-1(HIF-1),fluid shear stress and atherosclerosis,serotonergic synapse,and insulin resistance signaling pathways,to down-regulate neuroinflammation,prevent protein misfolding,relieve vascular nerve dysfunction,affect neurotransmitters,and regulate cell metabolism and mitochondrial function.Moreover,they can intervene in the early stage of disease and upstream region of the pathways.