基于顶空固相微萃取-气相色谱-三重四极杆质谱联用技术的余甘子挥发性成分分析及抗仓储害虫作用研究
CSTR:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(81973493);四川省中医药标准化研究项目(2019073);三勒浆药业-成中医联合实验室项目(2019-YF04-00086-JH)


Volatile Components of Phyllanthus emblica L.and Its Effect Against Storage Pests Based on HS-SPME-GC-QQQ-MS/MS Odor Analysis Technology
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:对余甘子挥发性成分进行富集分析,并评价其抗虫作用。方法:采用CO2超临界流体萃取法制备余甘子精油,采用顶空固相微萃取-气相色谱-三重四极杆质谱联用(HS-SPMEGC-QQQ-MSMS)技术构建了余甘子气味成分谱(OCS)和气味强度特征谱(OICS),对比余甘子药材粉末与精油的挥发性物质组成差异。在封闭空间内研究余甘子药材及精油对药材甲、咖啡豆象、米象、印度谷蛾等仓储害虫死亡率、进食率和趋避作用的影响。结果:药材和精油成分基本一致,但药材中小分子酸类等成分含量更高。余甘子药材与精油均对进食率影响不大,但能提高昆虫死亡率,趋避作用明显,且余甘子精油驱虫效果更强。结论:余甘子药材和精油具有一定的驱虫作用,乙酸乙酯、芳樟醇、α-松油醇等可能是其抗虫主要物质基础。本文首次报道了余甘子的抗虫作用,为余甘子精油的综合开发提供参考。

    Abstract:

    To enrich and analyze the volatile components of Phyllanthus emblica L.and evaluate its pest resistance.Methods:The essential oil of P.emblica L.was prepared by CO2 supercritical fluid extraction,and the odor component spectrum(OCS) and odor intensity characteristic spectrum(OICS) of P.emblica L.were constructed by headspace sampling-solid-phase microextraction-gas chromatography-triple quadrupole-mass spectrometry/mass spectrometry(HS-SPME-GC-QQQ-MS/MS) technology.The difference in volatile components between the powder of P.emblica L.and its essential oil was compared.The effects of P.emblica L.and its essential oil on the mortality,feeding rate,and evading of storage pests such as Stegobium paniceum,Araecerus fasciculatus,Sitophilus oryzae,and Plodia interpunctella were studied in an enclosed space.Results:The components of the crude drug and the essential oil were basically the same,but the content of small molecular acids and other components in the crude drug was higher.Both P.emblica L.and essential oil have little effect on the feeding rate,but they can increase the mortality of pests with obvious evasive effects.The effect of P.emblica L.essential oil on pest repellency is stronger.Conclusion:P.emblica L.and its essential oil have certain pest-repellent effects.Ethyl acetate,linalool,and α-terpineol may be the main material basis of its pest resistance.This paper reports the pest resistance of P.emblica L.for the first time and provides references for the comprehensive development of P.emblica L.essential oil.

    参考文献
    相似文献
    引证文献
引用本文

黄浩洲,张定堃,李梦琪,谭鹏,包晓明,莫太刚,樊三虎,韩丽,林俊芝.基于顶空固相微萃取-气相色谱-三重四极杆质谱联用技术的余甘子挥发性成分分析及抗仓储害虫作用研究[J].世界中医药,2022,(19).

复制
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-08-26
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2022-11-14
  • 出版日期:
文章二维码