To investigate the mechanism of salidroside in the cardiac dysfunction of rats with thermoplegia.Methods:Forty male rats in specific pathogen-free(SPF) grade were selected to construct models of thermoplegia，and the rats were divided into four groups according to the difference of intervention methods，namely，the control group，the low，medium，and high-dose salidroside groups(4 mg/kg，8 mg/kg，and 32 mg/kg)，with 10 rats in each group.The cardiac function damages of rats in the 4 groups were compared.Results:The higher the dose of salidroside，the higher the membrane potential，respiratory control rate，and membrane permeability，and the lower the oxygen free radicals in the rat heart(P<0.05).Malonaldehyde(MDA) in the salidroside groups was lower than that in the control group，and superoxide dismutase(SOD) was higher than that in the control group.The higher the dose of salidroside，the lower MDA and the higher SOD(P<0.05).The myocardial cells of rats in the control group were significantly swollen，and the mitochondrial damage of the myocardial cells of rats decreased with the increase of the dose of salidroside.The peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α) and manganese-SOD(MnSOD) mRNA in the salidroside groups were higher than those in the control group，and the higher the dose of salidroside，the higher the PGC-1α and MnSOD mRNA(P<0.05).Conclusion:Rhodiola rosea glycosides used in the intervention of rats with severe heat stroke helped to reduce the degree of cardiac impairment，which was important for the improvement of cardiac function and respiratory control rate,and achieve the effect of protecting heart function.The higher the dose,the more obvious the effect.The mechanism may be related to the up-regulation of PGC-1α and MnSOD mRNA.