To study the effects of different concentrations of berberine on the cloning,metastasis,and Toll-like receptor 4(TLR4) protein of laryngeal carcinoma cells.Methods:The laryngeal carcinoma cells were divided into a blank control group(K1 group),a 5 μmol/L berberine intervention group(K2 group),a 10 μmol/L berberine intervention group(K3 group),and a 20 μmol/L berberine intervention group(K4 group).Western blotting method was used to detect TLR4 and TLR2 protein levels,and the methyl thiazolyl tetrazolium(MTT) assay was used to detect cell proliferation.The scratch test was used to detect cell migration,and the flow cytometry was used to detect cell apoptosis.Results:At 24 h,the cell clone formation ability of the K1 group was similar to the K2 group(P>0.05),and the cell clone ability of the K1 group was higher than that of the K3 and K4 groups(P<0.05).At 48 h and 72 h,compared with the K1 group,the other three groups had a significant decrease in the colony formation rate(P<0.05).Moreover,the clone formation rate of the K4 group was significantly lower than that of the K3 and K2 groups(P<0.05).Compared with the K1 group,the K2,K3,and K4 groups decreased TLR2 and TLR4 proteins(P<0.05).Compared with the K2 and K3 groups,the K4 group decreased TLR2 and TLR4 proteins(P<0.05).Compared with the K1 group,the K2,K3,and L4 groups decreased cell proliferation rate and scratch distance,and increased apoptosis rate(P<0.01).As compared with the K2 group and K3 group,the cell proliferation rate and scratch distance of the K4 group decreased,and the apoptosis rate increased(P<0.05).Conclusion:Different concentrations of berberine can reduce the cloning,proliferation,and migration ability of laryngeal carcinoma cells,and the mechanism may be related to the inhibition of the activity of TLR2 and TLR4 proteins,thereby promoting the apoptosis of laryngeal carcinoma cells.