To investigate the effects of catalpol on glucolipid metabolism and its complications in type 2 diabetic db/db mice.Methods:The db/db mice were taken as a model of type 2 diabetes and randomly divided into model group，metformin group(positive) and catalpol low-，medium-and high-dose groups.The db/m mice were used as control group.After 8 weeks of treatment，the rat body weight，blood glucose，liver function，and retinal changes were detected，and hepatic bile acid was determined using ultra-performance liquid chromatography-quadrupole linear ion trap mass spectrometry(UPLC/Q-TRAP/MS).Results:Compared with the conditions in model group，catalpol regulated body weight，reduced blood glucose，improved glucose tolerance，decreased levels of serum alanine aminotransferase(GPT)，glutamic oxaloacetic aminotransferase(GOT)，total bilirubin(T-Bil)，albumin(ALB)，direct bilirubin(D-Bil) and indirect bilirubin(I-Bil) in db/db mice and their organ coefficient(P<0.05)，and ameliorated liver and retinal damage.It was also found that catalpol and metformin down regulated the level of ursodeoxycholic acid(UDCA)(P<0.05) and up regulated the levels of lithocholic acid(LCA)，deoxycholic acid(DCA) and chenodeoxycholic acid(CDCA)(P<0.05) as well as the contents of folic acid(FA) and vitamin B12(VB12) in the liver of diabetic mice.Conclusion:Catalpol may regulate glucose metabolism in type 2 diabetic mice by regulating hepatic bile acid，improving liver and retinal damage and lowering the risk of peripheral neuropathy.