To explore the medication rule of Chinese medicine in treating follicular dysplasia by data mining，and the effect of new Chinese medicine prescriptions obtained by cluster analysis on ovarian function in rats with ovulation disorder.Methods:Relevant Chinese and English literature in the past 20 years were retrieved.The data of Chinese medicine were extracted and sorted out，and the medication characteristics and association rules of Chinese medicine for the treatment of follicular dysplasia were analyzed using SPSS Modeler 18.0 and SPSS Statistics 24.0.New Chinese medicine prescriptions obtained by cluster analysis was determined for experimental research.Female SD rats were randomly divided into control group，model group(rat model of androgen-induced ovulation disorder)，Letrozole group(model group+Letrozole)，and Bushen Jianpi Prescription group(model group+Bushen Jianpi Prescription)，with 10 in each group.Hematoxylin and eosin(HE) staining was used to observe the development of follicles at all levels in the ovaries of rats，and enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of serum estradiol(E2)，luteinizing hormone(LH)，follicle stimulating hormone(FSH)，progesterone(P) and other sex hormones.Results:A total of 132 prescriptions were included in the study，involving 146 Chinese herbal medicines and treating 8 syndromes such as kidney deficiency and blood stasis syndrome and kidney yin deficiency syndrome.Among the Chinese herbal medicines，27 such as Radix Angelicae Sinensis，Radix Rehmanniae Preparata，and Semen Cuscutae were used more than 20 times.They were mainly sweet and pungent in taste and warm and mild in property，acting on kidney，followed by spleen and liver.The herbal pairs Radix Angelicae Sinensis-Radix Rehmanniae Preparata and Radix Rehmanniae Preparata-Semen Cuscutae had the highest reliability of association rules，and three new combinations were obtained by cluster analysis.After expert discussion，the new formula Bushen Jianpi Prescription was used for experimental research.The results showed that most of the follicles in the ovaries of the rats with androgen-induced ovulation disorder had cystic expansion，and there was almost no luteinization.In contrast，the number of developing follicles，granulosa cells and corpus luteum in the ovaries of the rats in the Bushen Jianpi Prescription group increased，and the levels of serum E2，P and FSH were up-regulated.Conclusion:Through data mining，Chinese medicines for tonifying kidney are mainly used in the treatment of follicular dysplasia.Bushen Jianpi Prescription obtained by cluster analysis improves the ovarian tissue morphology，promotes follicular development，and regulates serum hormone levels，thereby enhancing ovarian reserve function in rats.