To explore the efficacy of Yiqi Yangyin Prescription(YQYY) on hyperglycemic rats with qi and yin deficiency based on prescription-syndrome correspondence.Methods:A total of 48 male SD rats were divided into blank group，model group，positive drug(Shiwei Xiaoke Capsule) group，and YQYY low-，medium-and high-dose groups according to basal blood glucose，with 8 in each group.The hyperglycemic rat model of insulin resistance was established by thyroxine(sc)+continuous high-fat diet feeding+streptozotocin(STZ，ip).The drugs were administered for 30 consecutive days from the first day of the experiment.The general condition of the rats was observed，and the body weight was measured.The related indexes of glucose and lipid metabolism and the biological indexes related to qi and yin deficiency were detected.Results:Compared with the blank group，the model group presented qi and yin deficiency with increased thirst，urination and hunger as well as weight loss.And fasting blood glucose(FBG)，area under the curve(AUC)，homeostatic model assessment of insulin resistance(HOMA-IR)，total cholesterol(TC)，triglycerides(TG)，low-density lipoprotein cholesterol(LDL-Ch)，triiodothyronine(T3)，thyroxine(T4)，cyclic adenosine monophosphate(cAMP) and cAMP/cyclic guanosine monophosphate(cGMP) in the model group were higher(P<0.05，P<0.01)，while body weight，HOMA-β，insulin sensitivity index(ISI)，glycosylated hemoglobin(HbA1c)，fasting insulin(FINS)，high-density lipoprotein cholesterol(HDL-Ch)，cGMP，spleen index，thymus index，grip index，immunoglobulin G(IgG)，(IgM)，motilin(MTL) and gastrin(GAS) were lower(P<0.05，P<0.01) than the blank group.Compared with the conditions in the model group，qi and yin deficiency was alleviated in the YQYY groups，and body weight，spleen index，thymus index，FINS，HOMA-β，ISI，cGMP，MTL，GAS，IgG and IgM were increased(P<0.05，P<0.01)，while FBG，AUC，HOMA-IR，T3，T4，cAMP and cAMP/cGMP were decreased(P<0.05，P<0.01).The YQYY high-dose group had elevated grip index(P<0.01，P<0.05) while reduced TG，TC and LDL-Ch(P<0.05).The YQYY low-dose group had down-regulated TG while up-regulated HDL-Ch(P<0.05).Conclusion:YQYY relieves the syndromes of hyperglycemic rats with qi and yin deficiency and regulates the glucose and lipid metabolism disorders.