To observe the effect of taking methods of Besunyen Detox Tea(water addition and soaking times) on dissolution of anthraquinones and laxation.Methods:The content determination method of total anthraquinone was established by ultraviolet spectrophotometry(UV)，and that of sennoside A，sennoside B and rhein was established by high-performance liquid chromatography(HPLC)，to investigate the content variation of effective substances in Besunyen Detox Tea under different soaking times and water addition.The constipation model was constructed by loperamide to verify the laxative effect of Besunyen Detox Tea and explore its effect on intestinal motility and mucus secretion.The influence of different soaking times and water addition on propulsion rate of mouse small intestine，first melena time，melena times within 5 h，fecal water content，and colon motilin(MTL)，gastrin(GAS)，somatostatin(SS) and substance(SP) was also investigated.Results:Water addition was proportional to the dissolution of anthraquinones，and there was little difference in the dissolution of anthraquinones when the water addition was 60~100 times.Most anthraquinones were dissolved by soaking once，and from the second soaking，the contents of total anthraquinone，sennoside A，sennoside B and rhein were decreased significantly.The animal experiments revealed that when the water addition was 40 times，60 times and 100 times，Besunyen Detox Tea produced a laxative effect，and no adverse reactions such as diarrhea occurred.The laxative effect of Besunyen Detox Tea added with water of 60 times and 100 times was obvious.Moreover，water addition of 100 times and repeated brewing failed to be better than soaking once in laxative effect.Conclusion:This experiment provided a basis for precise application of the product and had positive significance for the standardization of anthraquinone products.