To explore the anti-liver fibrosis mechanism of Agrimoniae Herba.Methods:The chemical compounds of Agrimoniae Herba were screened out by multiple methods and the target genes were predicted and obtained.The potential targets of Agrimoniae Herba against liver fibrosis were obtained through the comparison with the targets related to the pathogenesis of liver fibrosis.Gene Ontology(GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis were carried out for associated targets through DAVID.The chemical compound-target-pathway interaction network was visualized by Cytoscape software.The effects of Agrimoniae Herba on the expression of α-smooth muscle actin(α-SMA) and collagen type 1 alpha 1(COL1A1) and the phosphorylation of protein kinase B(AKT) in rat hepatic stellate cell-T6(HSC-T6) were further verified by experiments.Results:The key targets were mainly AKT1，STAT3，caspase-3，Jun，and ESR1，which were mainly involved in the regulation of tumor signaling pathway，PI3K/AKT signaling pathway，tumor proteoglycans，insulin resistance，FoxO signaling pathway，etc.Agrimoniae Herba extract could inhibit the protein expression of α-SMA and COL1A1 and increase the phosphorylation level of AKT in HSC-T6，which verified the partial prediction results of network pharmacological analysis.Conclusion:Agrimoniae Herba could inhibit liver fibrosis by acting on the PI3K/AKT and other signaling pathways.