To investigate the prevention and treatment mechanism of Scutellariae Radix-Paeoniae Radix Alba on type 2 diabetes mellitus(T2DM) and find the relevant metabolic pathways by searching for endogenous potential biomarkers based on renal metabolomics technology.Methods:The non-diabetic healthy young mice born in the same litter(db/m mice) were assigned to the blank group，and the mice deficient in the diabetic leptin receptor gene with a blood glucose value higher than 11.1 mol/L(db/db) were divided into a model group，a Scutellariae Radix-Paeoniae Radix Alba group(5.625 g/kg)，and a positive drug group(metformin，250 mg/kg).The drug was administered once per day for eight weeks，with a volume of 7.5 mL/kg，and the ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS) method was used for the renal metabolomics analysis of mice to explore the potential biomarkers.The relevant metabolic pathways were identified by combining the human metabolome database(HMDB) and the Kyoto Encyclopedia of Genes and Genomes(KEGG).Results:The Scutellariae Radix-Paeoniae Radix Alba group could reduce the metabolic trajectory deviation of T2DM model mice.Meanwhile，14 biomarkers with common differences with DN model and after Huangqin Decoction intervention were identified，involving eight relevant metabolic pathways.Relevant metabolic pathway analysis methods were applied to find that the metabolic pathways with the most extensive influence might be glycerophospholipid metabolism，purine metabolism，and pyrimidine metabolism.Conclusion:The mechanism of Scutellariae Radix-Paeoniae Radix Alba in the treatment of T2DM might be attributed to improving the level of endogenous metabolites and restoring the normal metabolic activities of the body by regulating multiple metabolic pathways such as lipid metabolism and energy metabolism.