To evaluate the methodological and reporting quality of animal experimental studies on the treatment of ulcerative colitis with Sophorae Flavescentis Radix(SFR)-containing compound preparations.Methods:Relevant databases were searched from database inception to September 2021.Animal experimental studies on the treatment of ulcerative colitis with SFR-containing compound preparations were included.Two authors independently screened and extracted data from the literature and assessed the risk of bias and reporting quality of the included studies according to the Animal Research：Reporting of In Vivo Experiments(ARRIVE) 2.0 guidelines and the SYRCLE tool.Results:Sixteen studies were included，with 11 using SFR-containing compound preparations and 5 using Wuwei Kushen Enteric Capsules.According to the SYRCLE tool，one study reported the generation method of random sequence，baseline conditions，and blinding of outcome assessors，but all studies did not report allocation concealment，blinding of experimenters，and assessment of randomization results.According to the ARRIVE 2.0 guidelines，all studies did not report the method of sample size determination，inclusion and exclusion criteria，control strategy to minimize potential confounding factors，ethical review information，animal welfare during the experiment，establishment of humane endpoints for experimental animals，registration information of the experimental plan，data acquisition method，conflicts of interest，and the role of funding sources in study design，analysis，and reporting.Two studies explained how the animal species and models used in the experiment achieved the research objectives，and three studies reported adverse events that occurred during the study.Conclusion:The methodological and reporting quality of animal experimental studies on the treatment of ulcerative colitis with SFR-containing compound preparations included in this study is low.It is recommended that future animal experimental studies be rigorously designed and implemented，following the SYRCLE tool for scientifically reasonable design，and adhering to the ARRIVE 2.0 guidelines for the scientific and rigorous reporting of research results.