To observe the intervention effects of Bushen Yiqi Hexue therapy by Chinese medicine in in vitro fertilization-embryo transfer(IVF-ET) and explore the underlying mechanisms in improving IVF-ET pregnancy outcomes through the analysis of serum and follicular fluid reproductive hormones，cytokines，and related indicators.Methods:A total of 171 patients who met the research criteria and were admitted to Maternity and Child Health Care Hospital Hubei from January 2016 to June 2019 were randomly divided into a observation group(85 cases) and a control group(86 cases).Both groups underwent a short-long protocol，with the observation group additionally receiving Chinese medicine treatment for three months before oocyte retrieval.The serum and follicular fluid levels of reproductive endocrine hormones and related factors，uterine artery blood flow，and clinical pregnancy rates were observed at different time points in both groups.Results:The implantation rate and clinical pregnancy rate in the observation group were 54.43% and 68.23%，respectively，and 36.69% and 53.48% in the control group，respectively，and the observation group was superior to the control group(both P<0.05).Compared with the control group，the observation group had lower levels of human chorionic gonadotropin(hCG) on the day of injection and lower levels of estrogen(E2)，progesterone(P)，and testosterone(T) in the serum(P<0.05).On the day of oocyte retrieval，the observation group had lower E2 and P levels(P<0.05).On the day of ET，the E2 level was reduced，and on the 7th day after ET，the E2 level was higher in the observation group(both P<0.05).The observation group had higher levels of insulin-like growth factor-2(IGF-2)，interleukin-1β(IL-1β)，interleukin-6(IL-6)，leukemia inhibitory factor(LIF)，transforming growth factor-β1(TGF-β1)，and vascular endothelial growth factor(VEGF) in the serum on the day of oocyte retrieval and ET，while tumor necrosis factor-α(TNF-α) levels were lower(all P<0.05).On the 7th day after ET，the serum levels of IGF-2，IL-1β，IL-6，LIF，and VEGF were higher，and TNF-α levels were lower(all P<0.05).The observation group had a higher E2 level and a lower P level in the follicular fluid(both P<0.05)，as well as higher levels of IGF-2，IL-1β，IL-6，LIF，TGF-β1，and VEGF，and lower TNF-α levels(all P<0.05).Compared with the control group，the observation group showed significant improvement in uterine artery blood flow(P<0.05).Conclusion:Bushen Yiqi Hexue therapy by Chinese medicine significantly improves IVF-ET pregnancy outcomes，possibly through their regulation of serum and follicular fluid reproductive hormones，cytokines，and uterine artery blood flow.