To investigate the mechanism of Siwutang in the treatment of Alzheimer's disease(AD) based on network pharmacology.Methods:The chemical components of Siwutang were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)，and the corresponding targets were predicted.Cytoscape3.7.2 was used to build the component-target network.The targets of AD were obtained from GeneCards and Online Mendelian Inheritance in Man(OMIM).The protein-protein interaction(PPI) network was established，and the Gene Ontology(GO) annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment were performed.The prediction results were preliminarily verified by in vitro cell experiments.Results:A total of 19 active components and 46 potential targets of Siwutang were predicted.The PPI network showed that the core targets were protein kinase B1(AKT1)，caspase-3(CASP3)，Jun proto-oncogene(JUN)，tumor necrosis factor(TNF)，and prostaglandin-endoperoxide synthase 2(PTGS2).The enrichment results showed that the core targets were mainly involved in the biological processes such as the positive regulation of neuronal apoptosis and the cell apoptosis and Toll-like receptor signaling pathways.The cell experiments showed that Siwutang significantly up-regulated the expression of B-cell lymphoma-2(Bcl-2).Conclusion:Siwutang can treat AD by acting on multiple targets and pathways.This study summarizes the theoretical basis of application of Siwutang and preliminarily reveals the possible molecular mechanism，providing theoretical support for subsequent clinical application and experimental studies.