To observe the effects of flavonoids from Alpiniae Officinarum Rhizoma，Galangae Rhizoma，and Galangae Fructus on the levels of cyclic nucleotides and the sympathetic-adrenal axis in gastric ulcer rats with cold syndrome and explore the material basis for the warm and heat medicinal properties of these three Alpinia medicinal plants.Methods:The gastric ulcer model with cold syndrome was induced in rats by oral administration of iced Anemarrhenae Rhizoma decoction and 15% glacial acetic acid.Gingerol from Zingiberis Rhizoma was used as the positive control.Enzyme-linked immunosorbent assay(ELISA) was used to measure the levels of adenylate cyclase(AC)，phosphodiesterase 2(PDE2)，cyclic adenosine monophosphate(cAMP)，cyclic guanosine monophosphate(cGMP)，adrenocorticotropic hormone(ACTH)，and dopamine β-hydroxylase(D-β-H).Results:Compared with the blank group，the model group showed significantly reduced levels of AC，cAMP，and the cAMP/cGMP ratio in gastric tissues，as well as significantly increased levels of PDE2(P<0.01).Compared with the model group，the high-and low-dose Alpiniae Officinarum Rhizoma，Galangae Rhizoma，and Galangae Fructus groups showed increased levels of AC，reduced levels of PDE2，and increased levels of cAMP and the cAMP/cGMP ratio in gastric tissues(P<0.01 or P<0.05).Conclusion:Flavonoids from three Alpinia medicinal plants can promote the activity of the sympathetic-adrenal axis in gastric ulcer rats with cold syndrome by regulating the levels of cyclic nucleotides，reflecting the warm and heat properties of these flavonoids.