To study the clinical effect and mechanism of colon dialysis with Chinese medicine decoction on stage 3 to 4 chronic kidney disease(CKD) patients with dampness turbidity syndrome by using metabonomics.Methods:Sixty patients with stage 3 to 4 CKD(dampness turbidity syndrome) treated in the outpatient and ward of Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine were enrolled in this study.The patients were randomized into a control group and an observation group.On the basis of integrated treatment of chronic renal failure，the patients were treated with colon dialysis with Chinese medicine decoction.After treatment，the TCM syndrome scores，response rate，and biochemical indicators were measured to determine the efficacy.In addition，serum metabolomics was employed to analyze the metabolomics in the healthy volunteers and the patients.Univariate statistical analysis，multivariate statistical analysis，hierarchical cluster analysis of differential metabolites，and metabolic pathway analysis of differential metabolites were performed.Results:The response rate in the control group was 30.00%，significantly different from that(60.00%) in the observation group.The treatment in the observation group lowered the blood creatinine and urea nitrogen levels and increased the glomerular filtration rate(P<0.05).Moreover，the blood creatinine，urea nitrogen，and glomerular filtration rate had significant differences between the control and observation groups after treatment(P<0.05).A total of 6 biomarkers were identified，involving 2 main metabolic pathways.Conclusion:Colon dialysis with Chinese medicine decoction can increase the glomerular filtration rate，delay disease progression，and alleviate symptoms in patients with stage 3 to 4 CKD(dampness turbidity syndrome).