To explore and clarify the mechanism of Lingqi Mixture against the development of colorectal cancer through the hepatocyte growth factor/mesenchymal-epithelial transition factor(HGF/c-Met) signaling pathway.Methods:Rats with rectal cancer were divided into high-，medium-and low-dose Lingqi Mixture groups，and the therapeutic effects of traditional Chinese and Western medicines were compared.Western blot，immunofluorescence and apoptosis detection were proformed to observe and analyze the tumor growth，apoptosis and the protein expression of HGF/c-Met signaling pathway.Results:In Western blot detection，the protein expression of HGF in tumor tissue increased，while that of c-Met decreased; after the Lingqi Mixture treatment，the expression of HGF was down-regulated，while that of c-Met was up-regulated，which was consistent with the results of immunofluorescence，and the difference was significant(P<0.05).Conclusion:Lingqi Mixture can prevent and treat colorectal cancer by down-regulating the expression of HGF and up-regulating the expression of c-Met to inhibit the HGF/c-Met signaling pathway，thereby suppressing the invasion and metastasis of colorectal cancer cells.