To explore the mechanism of massage in reducing intraocular pressure in glaucoma by promoting the generation of aqueous humor prostaglandin and inducing the intraocular lymphatic hyperplasia in rats.Methods:Forty-eight SD rats were randomized into glaucoma model，massage，latanoprost，and massage+latanoprost groups(n=12).The aqueous humor samples were collected from each group at different time points during the treatment period for enzyme-linked immunosorbent assay.Western blotting，qPCR，immunofluorescence assay，and immunohistochemical staining were employed to determine the expression levels of prospero homeobox 1(Prox-1) and 5'-Nase.Histochemical staining was employ to detect the lymphatic hyperplasia in the ciliary body in each group.Results:The massage+latanoprost group had lower intraocular pressure(P<0.05)，higher expression levels of Prox-1 and 5'-Nase(P<0.05)，and higher rate of lymphatic hyperplasia in the ciliary body than other groups.Conclusion:Massage can promote the lymphatic hyperplasia in the ciliary body，which can facilitate the absorption of excess aqueous humor to reduce intraocular pressure.