Aiming at the problem that“nine”in“nine steamings and nine sun-dryings”is not clear，this paper explored the change rule in extracts and polysaccharides in the one to nine steamings of wine-processed Polygonati Rhizoma，and investigated the immune regulation effect of wine-processed Polygonati Rhizoma with different steaming times on immunosuppressed mice，to optimize the optimal steaming times．Methods:Taking appearance traits，extract content and polysaccharides as the evaluation indexes，the comprehensive scoring method was employed to select the top three decoction pieces of wine-processed Polygonati Rhizoma.Cyclophosphamide was used to establish a mouse immunosuppression model.The body weight change and immune organ index of the mice after administration of the three batches of wine-processed Polygonati Rhizoma were observed，and the function of phagocytic cells was analyzed by the clearance of carbon particles，to obtain the optimal steaming times of wine-processed Polygonati Rhizoma.Results:The comprehensive scores of four steamings and four sun-dryings，five steamings and five sun-dryings as well as six steamings and six sun-dryings of wine-processed Polygonati Rhizoma were the highest，which were 0.72，0.66 and 0.75，respectively.Compared with the model group，the body weight change in the groups of four steamings and four sun-dryings，five steamings and five sun-dryings and six steamings and six sun-dryings was increased(P<0.01，0.05)，and the thymus and spleen indices were elevated(P<0.01).The function of phagocytic cells in the three groups was also improved(P<0.01，0.05).There was no significant difference in the body weight change and thymus index between the three batches of wine-processed Polygonati Rhizoma，but the spleen index in the group of six steamings and six sun-dryings was higher than that in the group of four steamings and four sun-dryings(P<0.05)，and the correction index in the group of six steamings and six sun-dryings was higher(P<0.05)．Conclusion:The comprehensive scores of wine-processed Polygonati Rhizoma after six steamings and six sun-dryings are the highest in terms of appearance traits，extract and polysaccharides.According to the traditional process of four，five and six steamings，wine-processed Polygonati Rhizoma can regulate immunity，among which，six steamings and six sun-dryings has the most significant effect.Therefore，six steamings is the optimal for wine-processed Polygonati Rhizoma.It is advisable to make yellow essence with six steamings and six sun-dryings.