To study the inhibitory effect of Xiasangju Granules on human coronavirus-229E(HCoV-229E).Methods:The safe dose of Xiasangju Granules on A549 cells was detected by the cytopathic effect(CPE) method，and the effect of Xiasangju Granules on cytopathic effect of A549 cells caused by HCoV-229E was detected by the CPE method.Reverse transcription polymerase chain reaction(RT-PCR) method was used to detect the effects of Xiasangju Granules on the replication of HCoV-229E in A549 cells.The safe dose of Xiasangju Granules on Huh-7 cells was detected by Thiazolyl Blue(MTT) method，and RT-PCR method was used to detect the effects of Xiasangju Granules on the expression of inflammatory mediators in Huh-7 cells caused by HCoV-229E.Results:The median inhibitory concentration of Xiasangju Granules on CPE of A549 cells infected by HCoV-229E is 23.78 mg crude drug/mL，and the selection index is 2.0.Xiasangju Granules can dose-dependently inhibit the viral load of HCoV-229E in A549 cells and inhibit the mRNA overexpression of monocyte chemoattractant protein-1(MCP-1)，tumor necrosis factor-α(TNF-α)，interleukin-6(IL-6)，and interleukin-8(IL-8) induced by HCoV-229E in Huh-7 cells Conclusion:Xiasangju Granules have obvious anti-infection effects of HCoV-229E in vitro.