To explore the anti-inflammatory mechanisms of acacetin in lipopolysaccharide(LPS)-induced mouse bone marrow-derived macrophages(BMDMs).Methods:The effect of various concentrations of acacetin(5，10，20，and 40 μmol/L) on the proliferation of BMDMs was tested using the CCK-8 method，so as to select the optimal dosage.BMDMs were isolated and cultured，and LPS(50 ng/mL) was used to pretreat BMDMs for 0，10，30，and 60 minutes.Then，BMDMs were added with acacetin(10 μmol/L) and cultured for 0.5 h.Western blotting was used to analyze the expression of phosphorylated p65(p-p65)，p65，nuclear factor(NF) κB suppressor protein α(IκBα)，and phosphorylated IκBα(p-IκBα).The nuclear translocation of NF κB was detected by laser scanning confocal microscope(LSCM).Results:CCK-8 results showed that acacetin concentrations ranging from 5 to 40 μmol/L had no significant effect on the proliferation of BMDMs.Based on previous studies of the team，10 μmol/L of acacetin was chosen for subsequent experiments.Compared with the blank control group，LPS stimulation significantly increased the expression levels of p-p65 and p-IκBα.Treatment with acacetin significantly reduced the expression levels of p-NF-κB p65 and p-IκBα.Moreover，acacetin inhibited the LPS-induced nuclear translocation of NF-κB p65.Conclusion:The anti-inflammatory effects of acacetin were related to the inhibition of the NF-κB signaling pathway，suggesting acacetin may be a potential anti-inflammatory drug.