To analyze the molecular mechanism of Dingchuan Decoction in the treatment of chronic obstructive pulmonary disease(COPD) and verify the therapeutic effect of Dingchuan Decoction through cell experiments.Methods:In the TCMSP database，the active components of nine kinds of traditional Chinese medicine(TCM) in Dingchuan Decoction were screened，and the relevant targets of active components of TCM were obtained by the Uniport database.Then，the target genes corresponding to the diseases were collected by searching the keyword COPD in the five disease databases.Topological analysis was carried out on the network of active components-intersection targets，and the key components of Dingchuan Decoction in treating COPD were selected according to the connection between active components of TCM and intersection targets.Finally，the therapeutic mechanism of Dingchuan Decoction was determined by gene ontology(GO) enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG) enrichment analysis，and molecular docking was carried out.The immune and inflammatory mechanism of Dingchuan Decoction in treating COPD was clarified from the perspective of the whole biological network，and it was verified by cell experiment.Results:A total of 274 active components in Dingchuan Decoction and 262 disease-related intersection targets were screened through the experiment.KEGG enrichment analysis showed that the core target acted on the interleukin-17(IL-17) signaling pathway，NOD-like receptor signaling pathway，chemokine signaling pathway，and other immune and inflammatory signaling pathways.Molecular docking showed that the core compound had good docking activity with the target.Cell experiments showed that Dingchuan Decoction could inhibit the inflammatory reaction of human type Ⅱ alveolar epithelial cells through the PI3K/AKT signaling pathway，which confirmed the action target of network pharmacology.Conclusion:Through the preliminary screening and prediction of network pharmacology，the target of Dingchuan Decoction in the treatment of COPD has been preliminarily defined，and the multi-channel action mechanism of Dingchuan decoction has been confirmed.Its active components can regulate the inflammatory response through the PI3K/AKT signaling pathway，so as to treat COPD，which points out a direction for subsequent in-depth research.