To explore the pharmacokinetics characteristics of Ruyi Zhenbao Tablets in the plasma，a classic Tibetan medicine，after repeated administration，so as to provide a reference and basis for clinical application.Methods:Six male and six female SD rats were divided into the control group and the administration group.They were administered for seven days.Blood samples were collected from their eyes at 0 min before the last administration and 0.25，0.5，0.75，1，2，4，8，24，and 48 h after the administration，and the pharmacokinetic parameters of agarotetrol，isoliquiritigenin，piperine，and ferulic acid in the plasma were determined.Results:Most of the peak time of the pharmacokinetics parameters of agarotetrol，isoliquiritigenin，piperine，and ferulic acid in the plasma were 1 to 8 h，0.25 h，0.25 to 8 h，and 0.25 h respectively.Cmax was 140±53.9，8.31±6.34，33.4±22.2，and 15.7±7.90 ng/mL，respectively.t1/2 was 10.1±3.85，8.51±4.23，4.45±1.35，and 67.3±55.1 h，respectively.AUC0-t was 1 890±1 440，45.6±14.3，288±150，and 443±165 h × ng/mL，respectively.MRT0-∞ was 13.2±7.45，9.80±4.27，4.91±1.34，and 103±77.1 h，respectively.The main pharmacokinetics parameters of agarotetrol，isoliquiritigenin，and piperine in rats did not show significant statistical significance(P>0.05)，while the main pharmacokinetics parameters of ferulic acid(Cmax and AUC0-t) showed significant statistical significance(P<0.05).Conclusion:This study established a research method for the pharmacokinetics of four components，namely agarotetrol，isoliquiritigenin，piperine，and ferulic acid，after repeated administration of Ruyi Zhenbao Tablets and analyzed their pharmacokinetics characteristics to guide the clinical administration cycle and interval.