To explore the effects of Guashi Decoction combined with Danggui Shaoyao Powder on sex hormones and endometrial inflammatory factors in mice models with polycystic ovary syndrome combined with insulin resistance(PCOS-IR).Methods:PCOS-IR mice models were constructed with letrozole and a high-fat diet，and the success of the model was evaluated by observing changes in the estrous cycle and measuring fasting blood glucose and fasting insulin in mice.The mice were randomly divided into a control group，model group，and traditional Chinese medicine(TCM) group，and the TCM was given Guashi Decoction combined with Danggui Shaoyao Powder by gavage after successful modeling，while the control group and model group were given an equal volume of pure water.Serum follicle-stimulating hormone(FSH)，luteinizing hormone(LH)，and total testosterone(T) levels were measured after 21 days of intervention by enzyme-linked immunosorbent assay(ELISA) method，and the levels of tumor necrosis factor-α(TNF-α)，interleukin-1β(IL-1β)，and interleukin-6(IL-6) in endothelial tissue were measured.Results:After modeling，the mice in the control group had a normal estrous cycle，while the mice in the model group had a disturbed estrous cycle，mostly in the interestrous phase.The homeostasis model assessment-insulin resistance(HOMA-IR) of the control mice was less than 2.69，while that in the model group was greater than 2.69.After 21 days of the administration，the serum LH，T，and LH/FSH of mice in the TCM group were lower than those in the model group(P<0.05)，and the mRNA and protein expression levels of TNF-α，IL-1β，and IL-6 in endothelial tissue were lower than those in the model group(P<0.05).Conclusion:Guashi Decoction combined with Danggui Shaoyao Powder can improve the serum hormone level，reduce the level of endothelial inflammatory factors，and improve the endothelial inflammatory status in PCOS-IR mice.