To study the skin whitening effect of Qibai powder and predict its potential mechanism by combining network pharmacology and molecular docking technology.Methods:Ultraviolet B band(UVB) irradiation was used to induce skin pigmentation on the guinea pig to establish the animal model.The whitening effect of Qibai powder on model guinea pigs was observed，and the number of melanocytes and biochemical indicators related to skin pigmentation，such as medium superoxide dismutase(SOD)，malondialdehyde(MDA)，tyrosinase(TYR) and lipofuscin(LF)，were also detected.The mechanism of whitening of Qibai powder was predicted using network pharmacology and validated by molecular docking and surface plasmon resonance(SPR).Results:In animal experiments，compared with the model group，Qibai powder significantly increased the brightness of the skin and reduced the melanocyte number，TYR，LF，and MDA levels，while significantly increasing SOD level.The network pharmacology study found that Qibai powder may act on key targets such as protein kinase B α(AKT1)，tumor necrosis factor(TNF)，and epidermal growth factor(EGFR) through key components such as catechin gallate，pachymic acid C，and alloimperatorin and mainly regulate the PI3K-AKT and TNF signaling pathway to reduce melanin deposition to achieve the whitening effect.The molecular docking and SPR results showed that the key components have good affinities with the key targets.Conclusion:Qibai powder can achieve the skin whitening effect through antioxidants and inhibiting the activity of TYR.In addition，Qi Bai powder can reduce melanin deposition and exerts its whitening effects by acting on multi-targets through multi-ingredients and regulating multiple pathways.It reflects the synergistic effects of traditional Chinese medicines，as well as the characteristics of multi-components，multi-targets，and multi-pathways.