To provide a reference for the clinical application and development of fresh medicines by statistically analyzing the medicines for fresh use and their application characteristics in the Materia Medica Arranged According to Pattern(Zheng Lei Ben Cao).Methods:The Chinese medicines included in this book were screened，and the information of the included fresh medicines was organized in an Excel sheet for statistical analysis.Results:A total of 296 fresh medicines were found in the Materia Medica Arranged According to Pattern(Zheng Lei Ben Cao)，including 234(79.05%) fresh herbal medicines and 62(20.95%) fresh animal medicines.The fresh medicines were mainly cold or cool.The fresh herbal medicines were mainly sweet，bitter，and pungent，and the fresh animal medicines were mainly sweet，salty，and bitter.Most fresh medicines(191，82.33%) were non-toxic.In terms of the usage，there were 337(53.32%) records of external application.As for the indications，there were 201(30.04%) records of surgical diseases.Conclusion:The Materia Medica Arranged According to Pattern(Zheng Lei Ben Cao) specifies the clinical usage of a variety of fresh herbal and animal medicines.However，the application of fresh medicines are limited，and efforts should be made to enhance the development and experimental studies of fresh medicinal resources.