To explore the mechanism of Zhuang ethnomedicine Qingdu Shenjin Formula in the treatment of rheumatoid arthritis(RA) through network pharmacology.Methods:The intersection targets of Qingdu Shenjin Formula and RA were identified using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)，the Online Mendelian Inheritance in Man(OMIM) database，and the GeneCards database.Major active components were obtained using network visualization and analysis software(Cytoscape)，and core targets were calculated using the normalized cross-correlation(NCC) method.Gene Ontology(GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis were conducted on the core targets.Molecular docking was used to analyze the binding affinity between major active components and core targets.The collagen-induced arthritis(CIA) rat model was constructed to verify the concentration of major proteins.Results:A total of 145 active components and 238 drug targets were identified，with 151 intersection targets.Enrichment analysis indicated that the intersection targets were involved in 19 GO terms and 75 signaling pathways，including the advanced glycation end products(AGEs)-receptor for AGEs(RAGE) signaling pathway and the tumor necrosis factor(TNF) signaling pathway.The main active components were quercetin，anthocyanin，stigmasterol，luteolin，and β-sitosterol.Molecular docking results showed good binding affinity between these components and proteins such as prostaglandin-endoperoxide synthase 2(PTGS2)，matrix metalloproteinase 9(MMP9)，and serine/threonine kinase(AKT1).Animal experiments confirmed that Qingdu Shengjin Formula significantly reduced joint swelling in CIA rats and lowered serum concentrations of MMP9，AKT1，and PTGS2.Conclusion:Qingdu Shengjin Formula exerts its effects on the inflammatory cascade reaction of RA through multiple components，multiple targets，and multiple pathways.