To compare the content differences of eight flavonoid components in different processed products of Scutellariae Radix(SR) in rats and to explore the differences of pharmacokinetic behaviors of these products,providing scientific evidence for elucidating the processing principles.Methods:Twenty SD rats were randomly divided into control group,raw SR group,wine-processed SR group,and carbonized SR group,with five rats in each group.Drugs were administered by gavage.Using kaempferol as an internal standard,ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-QQQ-MS/MS) was employed to measure the concentrations of baicalin,5,7,2',5'-tetrahydroxy-8,6'-dimethoxyflavone(HQ-6),wogonoside,baicalein,wogonin,chrysin,5,6-dihydroxy-7,8,2',6'-tetramethoxyflavone(HQ-2),and oroxylin A in rat serum at various time points.The area under the curve(AUC0→∞) percentage of each component was used as a custom weighting coefficient to calculate the overall serum concentration of the drugs in rats and establish an integrated pharmacokinetic model to compare the differences in pharmacokinetic parameters of flavonoid components in processed products of SR.Results:The AUC0→∞ for single components varied significantly.The integrated pharmacokinetic results of multiple components showed that after wine processing of SR,the mean retention time(MRT0→∞) of the eight flavonoid components was reduced from 17.22 h to 13.13 h.The time to reach peak concentration(Tmax) decreased from 0.50 h to 0.25 h,while the time to secondary peak increased from 4 h to 8 h.After carbonization of SR,the overall MRT for the carbonized product was 10.07 h,which was not significantly different from the wine-processed product(P>0.05).The carbonized SR showed three peaks at 0.25,1,and 2 h.Conclusion:This method is specific and highly sensitive,suitable for simultaneous detection and pharmacokinetic studies of eight flavonoid components in rat serum,further elucidating the scientific meaning of “enhanced efficacy by wine processing and stability by carbonization' for SR.