To evaluate the effects of Yizhi Qingxin Formula on memory dysfunction in mice.Methods:After 15 days of administration of Yizhi Qingxin Formula，96 ICR mice were selected before training，after training，and before testing to establish mouse models of memory acquisition impairment，memory consolidation impairment，and memory retrieval impairment using scopolamine，sodium nitrite，and ethanol，respectively，with 32 mice in each model.Mice were randomly divided into control group，model group，low-dose Yizhi Qingxin Formula group，and high-dose Yizhi Qingxin Formula group，with 8 mice in each group.The open field test，novel object recognition test，and step-down test were used to assess the motor ability of the mice，as well as the effects of Yizhi Qingxin Formula on memory acquisition，consolidation，and retrieval impairments.Additionally，hematoxylin and eosin(HE) staining was used to observe the effects of Yizhi Qingxin Formula on the brain histopathology of mice with learning and memory impairment.Results:The open field test showed no significant difference in motor ability among the groups.The novel object recognition test indicated that the preference index and recognition coefficient for the new object were significantly higher in the low-dose and high-dose Yizhi Qingxin Formula groups than in the model group.The step-down test showed that the number of errors decreased，and the latency to avoid the platform increased in both the low-dose and high-dose Yizhi Qingxin Formula groups compared to the model group.HE staining of the hippocampus revealed that，compared to the results in the model group，the pyramidal cell layers in the low-dose and high-dose Yizhi Qingxin Formula groups were more intact，with clear stratification，tightly arranged cells，larger neurons，regular morphology，clear structure，and transparent cytoplasm.Conclusion:Yizhi Qingxin Formula can improve the learning and memory abilities of animals with memory dysfunction.