To investigate the protective effects of Morinda officinalis polysaccharides(MOP) on the testes of rats with varicocele(VC).Methods:A VC model was established using the left renal vein constriction method.Sixty rats were randomly divided into a NC group，a model group，a VC group，a VC+100 mg/kg MOP group，a VC+200 mg/kg MOP group，and a VC+300 mg/kg MOP group.Genes related to testicular repair in VC were screened using the GeneCards database.Hormone levels of gonadotropin-releasing hormone(GnRH)，testosterone(T)，luteinizing hormone(LH)，and follicle-stimulating hormone(FSH) were measured using enzyme-linked immunosorbent assay.Indicators related to the left testis and reactive oxygen species(ROS) levels were assessed.TUNEL staining was performed to detect apoptosis，and Western blot was used to measure the expression of phosphatase and tensin homolog deleted on chromosome 10(PTEN)，phosphoinositide 3-kinase(PI3K)，and protein kinase B(AKT) proteins.Results:Compared with the model group，the VC group exhibited significantly increased testicular interstitial area，apoptosis rate，ROS，GnRH，LH，FSH，and expression levels of PI3K，AKT，and p-AKT，and significantly decreased testicular-epididymal coefficient，seminiferous tubule diameter，epididymal sperm count，T，and PTEN protein expression(P<0.05).In the VC+100 mg/kg MOP group，testicular interstitial area，apoptosis rate，ROS，FSH，and expression levels of PI3K，AKT，and p-AKT were significantly reduced，while the testicular-epididymal coefficient，seminiferous tubule diameter，epididymal sperm count，T，and PTEN expression were significantly increased compared with the results in the VC group(P<0.05).In contrast，the VC+200 mg/kg MOP and VC+300 mg/kg MOP groups showed opposite results(P<0.05).Conclusion:MOP may repair the testes in VC rats by regulating the PTEN/PI3K/AKT signaling pathway.