To investigate the therapeutic effect of modified Dabuyin Pills on premature ovarian insufficiency(POI) with kidney yin deficiency syndrome.Methods:A total of 60 POI patients with kidney yin deficiency syndrome，treated at the Department of Gynecology，The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine(TCM) from October 2021 to June 2022，were enrolled and randomly divided into an observation group and a control group according to a random number table，with 30 patients in each group.The control group received hormone replacement therapy，while the observation group received modified Dabuyin Pills in addition to the treatment in the control group for three menstrual cycles.Changes in ovarian function and improvement in TCM syndrome scores before and after treatment were observed in both groups.Results:There were no significant differences between the two groups in estradiol(E2)，follicle-stimulating hormone(FSH)，luteinizing hormone(LH)，anti-Müllerian hormone(AMH)，antral follicle count(AFC)，peak systolic velocity(PSV)，resistance index(RI)，pulsatility index(PI)，and TCM syndrome scores before treatment(P>0.05).After treatment，both groups showed significant reductions in FSH，LH，RI，PI levels，and TCM syndrome scores compared to those before treatment(P<0.01)，with the observation group showing lower values than the control group(P<0.05).E2，AMH，AFC，and PSV levels significantly increased in both groups(P<0.01)，with higher levels in the observation group(P<0.05).The clinical effective rate was 93.33% in the observation group，higher than 73.33% in the control group(P<0.05).No adverse drug reactions were reported in either group.Conclusion:Modified Dabuyin Pills can effectively improve hormone levels and ovarian blood supply in POI patients with kidney yin deficiency syndrome to promote ovarian function recovery and significantly alleviate TCM symptoms，demonstrating definite therapeutic efficacy.