To explore the effects of electroacupuncture combined with rehabilitation training on knee joint function，three-dimensional gait，and inflammatory markers in patients with knee osteoarthritis(KOA) of cold-dampness obstruction syndrome.Methods:A total of 162 KOA patients admitted to Huadong Hospital，Fudan University from January 2021 to May 2023 were enrolled and randomly divided into an electroacupuncture group and a control group，with 81 patients in each group.The control group received routine rehabilitation training，while the electroacupuncture group received electroacupuncture treatment in addition to the same rehabilitation training，both for four weeks.The efficacy，symptom scores，Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)，Lysholm Knee Score(LKS)，three-dimensional gait parameters，and levels of inflammatory markers were compared between the two groups.Results:After treatment，the total effective rate in the electroacupuncture group was 92.59%，significantly higher than 77.78% in the control group(P<0.05).The electroacupuncture group showed lower TCM symptom scores(P<0.05)，lower WOMAC scores，and higher LKS scores than the control group(P<0.05).Gait parameters，including step frequency，speed，stride length，initial ground contact flexion angle，maximum swing phase extension angle，and support phase extension angle，were all higher in the electroacupuncture group.Additionally，the sagittal plane maximum abduction moment was lower in the electroacupuncture group.Inflammatory markers showed that interleukin-6 and tumor necrosis factor-α were lower in the electroacupuncture group，while transforming growth factor-β1 was higher(P<0.05).Conclusion:Electroacupuncture combined with rehabilitation training effectively enhances clinical efficacy，alleviates symptoms，improves knee joint mobility and walking ability，enhances knee function scores，and reduces inflammatory levels，contributing to the rapid recovery of knee joints in KOA patients.