To observe the effects of medicinal cake-separated moxibustion on the growth of ectopic endometrial tissue in a mouse model of endometriosis(EMs) and the expression of urokinase-type plasminogen activator(uPA)，matrix metalloproteinase 9(MMP-9)，E-cadherin，Axin，glycogen synthase kinase 3β(GSK3β)，and β-catenin，and to explore the mechanism of medicinal cake-separated moxibustion in inhibiting the adhesion and reconstruction of ectopic endometrial tissue in EMs.Methods:Forty-two female Balb/c mice were randomly divided into six groups：normal group，model group，medicinal cake-separated moxibustion group，Wnt agonist group，and Wnt inhibitor group.An autologous transplantation method was used to establish the EMs mouse model.Treatment was administered for 14 consecutive days，starting two weeks after surgery.At the end of the treatment period，the number of body twists was recorded，and the size of ectopic endometrial tissue was measured.Western blot was used to detect the expression of uPA，MMP-9，E-cadherin，Axin，GSK3β，and β-catenin in ectopic endometrial tissue.Results:Compared with the model group and Wnt agonist group，the medicinal cake-separated moxibustion group showed a significant reduction in the number of body twists and a significant decrease in the size of ectopic vesicles(P<0.05).Western blot results indicated that the protein expression of Axin，GSK3β，β-catenin，uPA，and MMP-9 in the medicinal cake-separated moxibustion group was significantly lower than that in the model and Wnt agonist groups(P<0.05).Moreover，the protein expression of E-cadherin was significantly higher in the medicinal cake-separated moxibustion group than in the model and Wnt agonist groups(P<0.05).Conclusion:Medicinal cake-separated moxibustion may reduce the expression of β-catenin，Axin，GSK3β，uPA，and MMP-9，and increase the expression of E-cadherin in endometrial tissue.It mediates the “adhesion-invasion-angiogenesis” developmental mechanism of ectopic endometrial tissue，inhibits its adhesion，and prevents the reconstruction of the extracellular matrix and basement membrane，thereby alleviating dysmenorrhea and inhibiting the growth of ectopic endometrial tissue in EMs mice.