To investigate whether the yeast extract-induced hyperuricemia model was accompanied by the syndromes of intrinsic dampness-heat and study the effect of Liqingtong Granules in lowering blood uric acid and clearing dampness-heat.Methods:The model was established by intragastric administration of 7.5 g/kg yeast extract daily.The contents of serum uric acid(SUA),creatinine(Scr),urea nitrogen(BUN),glutamic-pyruvic transaminase(GPT),and glutamic-oxaloacetic transaminase(GOT) were detected by the automatic biochemical analyzer.Serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-2(IL-2),interleukin-6(IL-6),aquaporin 3(AQP3),aquaporin 4(AQP4),motilin(MTL),gastrin(GAS),and aldosterone(ALD) were detected by enzyme linked immunosorbent assay(ELISA).The contents of xanthine oxidase(XOD) and adenosine deaminase(ADA) in serum were determined by colorimetry.Western blot assay was used to detect the protein expression levels of urate transporter 1(URAT1),glucose transporter 9(GLUT9),adenosine triphosphate binding cassette transporter G2(ABCG2),and organic anion transporter 1(OAT1) in the kidney,as well as GLUT9 and ABCG2 in the small intestine.Results:Compared with those of the blank group,the levels of SUA,GOT,Scr,MTL,and ADA in the serum,as well as URAT1,XOD,TNF-α,IL-1β,IL-6,ALD in the kidney and XOD levels in the liver of the model group were increased(P<0.05,P<0.01,P<0.001).ABCG2 in the kidney,ABCG2 in the small intestine,and IL-2,GAS,AQP3,and AQP4 levels in the small intestine decreased(P<0.05,P<0.01,P<0.001).Compared with the model group,the contents of SUA and ADA in serum,URAT1 in the kidney,as well as GOT,IL-1β,and MTL in serum of all groups of Liqingtong Granules were decreased(P<0.05,P<0.01,P<0.001).GAS content increased(P<0.001).The contents of serum XOD,TNF-α,IL-6,and ALD in the low-dose group were decreased(P<0.05,P<0.01); AQP3 content increased(P<0.05).The contents of XOD in the liver,GLUT9 in the small intestine,and serum XOD,TNF-α,ALD,and IL-6 decreased in the high-dose group(P<0.05,P<0.001).The levels of AQP4,IL-2,and AQP3 increased(P<0.01,P<0.001).Conclusion:The yeast extract-induced hyperuricemia model is accompanied by the syndrome of intrinsic dampness-heat,and Liqingtong Granules have the effect of lowering blood uric acid and clearing dampness-heat.