To explore the effects of Spatholobi Caulis on wound healing in diabetic cutaneous ulcers(DCU) in db/db mice and its underlying mechanism.Methods:Ten db/m mice were assigned to the blank control group.The db/db mice were used to establish a DCU model and randomly divided into the model group，positive control group，and Spatholobi Caulis group according to the random number table method，with 10 mice per group.From day 0，the blank and model groups were given distilled water(10 mL/kg) by gavage，with a gelatin sponge soaked in 50 μL of normal saline applied to the wounds.The positive control group received distilled water(10 mL/kg) by gavage，with a gelatin sponge soaked in 50 μL of bFGF applied to the wounds.The Spatholobi Caulis group received Spatholobi Caulis water extract(10 mL/kg，corresponding to 3.86 g/kg·d) by gavage，with a gelatin sponge soaked in 50 μL of normal saline applied to the wounds.Gavage was performed once daily，and wound dressing changes were done every other day，with wound photos taken.On day 21，the mice were euthanized，and the wound tissues were collected.Body weight，blood glucose，and wound healing rate were measured.Hematoxylin-eosin(HE) staining and Masson's trichrome staining were performed to observe the histopathological changes in the wound tissue.Network pharmacology and molecular docking were used to predict the potential pathways and targets of Spatholobi Caulis in treating DCU.Serum oxidative stress indicators(MDA and SOD) were measured using a microplate method.RT-qPCR was used to detect the mRNA expression of HIF-1α and VEGFA in the wound tissue.Immunohistochemistry and Western blot were used to detect the protein expression of HIF-1α and VEGFA in the wound tissue.Results:On days 0 and 21，compared to the blank group，the model group showed higher body weight and blood glucose(P<0.05).No significant difference was found between the model，positive control，and Spatholobi Caulis groups for body weight and blood glucose(P>0.05).On days 8，14，and 21，the model group had a lower wound healing rate than the blank group(P<0.05).The positive control and Spatholobi Caulis groups showed a higher wound healing rate than the model group(P<0.05).Histopathological changes in the wound tissue were improved in the positive control and Spatholobi Caulis groups compared to the model group.Seventeen key components of Spatholobi Caulis against DCU and 68 “drug-disease” common targets were identified，with core targets including HIF-1α and VEGFA.Molecular docking showed the highest binding energy between HIF-1α and aloe-emodin.Compared to the blank group，the model group had higher serum MDA levels(P<0.05) and lower SOD levels(P<0.05).No significant difference in MDA and SOD levels was observed between the positive control group and the model group(P>0.05)，but the Spatholobi Caulis group had lower MDA levels(P<0.05) and higher SOD levels(P<0.05).Compared to the blank group，the model group had lower mRNA expression of HIF-1α and VEGFA in the wound tissue(P<0.05).In comparison to the model group，the positive control and Spatholobi Caulis groups had higher mRNA expression of HIF-1α and VEGFA(P<0.05).The protein expression of HIF-1α and VEGFA in the wound tissue was lower in the model group than the blank group(P<0.05)，and higher in the positive control and Spatholobi Caulis groups compared to the model group(P<0.05).Conclusion:Spatholobi Caulis can promote wound healing in DCU mice by inhibiting oxidative stress and activating the HIF-1α/VEGFA pathway.